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Samples were allowed to cool, and 1.375 ml purified water with a resistivity 18.2 M cm from a Milli-Q system (Merck Millipore) was added to make up to a final volume of 1.5 ml

Factors contributing to the suboptimal performance of drugs include the selection of drug targets, the use of biomarkers and animal models in experimental designs, and other issues such as late treatment initiation, dose-dependent side effects, challenges in BBB permeability, and the heterogeneous presentation of patients

Biomedical materials for wound dressing: recent advances and applications